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    • 专利摘要:
    The present invention relates to the production of chemokine-inhibiting salicylic or nicotinic acid derivatives, their preparations and their uses in cosmetic or pharmaceutical compositions for preventing or treating internal and / or topical chronic inflammatory conditions.
    公开号:FR3026011A1
    申请号:FR1402163
    申请日:2014-09-24
    公开日:2016-03-25
    发明作者:Philippe Bernard；Dayana Abboud；Jean-Luc Galzi；Nelly Frossard；Christelle Pillard；Quoc Tuan Do
    申请人:Centre National de la Recherche Scientifique CNRS；Universite de Strasbourg；Greenpharma SAS；
    IPC主号:
    专利说明:

    [0001] The present invention relates to the production of certain chemo-attractant cytokine inhibiting derivatives (generally referred to as chemokines), their preparation and their uses in cosmetic or pharmaceutical compositions. Specifically, it relates to novel applications of said salicylic or nicotinic acid derivatives for treating chronic inflammatory diseases; in this respect, mention may be made of 10 chronic inflammatory external diseases such as dermatoses (acne, atopic dermatitis, psoriasis, eczema, dry skin with atopic tendency, redness. ") or internal ones such as Crohn's disease, asthma and rhinitis. Crohn's disease is a chronic inflammatory disease of the intestine The publication of Grip O. and Janciauskiene S. (Atorvastatin reduces plasma levels of chemokine (CXCL10) in patients with Crohn's disease, PloS One, 2009, 4 ( 5): e5263) shows the importance of chemokines in this pathology Allergic rhinitis is a chronic disease often associated with chronic dermatoses An article by Takeuchi S. et al (Changes in thymus and activationregulated chemokine (TARC) associated with allergen immunotherapy in patients with perennial allergic rhinitis 25 J Investigative Allergol Clin Immunol, 2005, 15 (3), 172-6) shows the involvement of chemokines in this pathology. skin and mucous membranes, which are characterized by unsightly manifestations such as redness and desquamation plaques. Several pathologies are grouped under the denomination of dermatoses. There may be mentioned, as non-limiting examples, eczema, atopic dermatitis, psoriasis, seborrheic dermatitis or acne.
    [0002] These dermatoses often result from inflammatory phenomena and immune disorders. In the case of atopic dermatitis, lesions are characterized by severe cutaneous dryness and inflammatory lesions: erythematous, papular, vesicular, squamous and very itchy eruptions (itching). Histologically, atopic dermatitis, like many dermatoses, is characterized by an infiltration of lymphocytes, monocytes and eosinophilic polynuclear cells around small vessels and capillaries. Biochemically, it has been shown that chemokines are strongly implicated in dermatosis and in chronic inflammatory diseases in general. For example, the publication of Folsgaard et al (Cord blood Th2-related chemokine CCL22 levels associated with elevated total-IgE during preschool age, Clin, Exp Allergy, 2012, 42 (11), 1596-603) sets evidence of the importance of the chemokine CCL22 in atopic dermatitis. Another publication by Hashimoto et al (Macrophage-derived chemokine (MDC) / CCL22 produced by monocyte derived dendritic cells reflects the disease activity in patients with atopic dermatitis, Journal of Dermatological Science, 2006, 44 (2), 93-9) states that highlight the importance of the same chemokine CCL22 in skin diseases.
    [0003] Chemokines are a family of small, mostly soluble, 8-14 kD proteins. Their most studied function is the attraction and control of the activation state of the cells of the immune system. Some chemokines have proven to be good therapeutic targets for treating internal and external chronic inflammatory diseases in humans or animals. The present invention highlights novel cosmetic, pharmaceutical and veterinary applications of certain effective agents for directly inhibiting chemokines and treating chronic inflammatory diseases in humans or animals. In this patent application, the generic term "pharmaceutical" is used to define both a product intended for application in humans and a product intended for application in animals. The subject of the invention is a cosmetic or pharmaceutical dermo composition that can be used for treating inflammatory diseases of human or animal skin, characterized in that it contains, in a pharmaceutically acceptable vehicle, an effective amount of at least one compound of formula (I): wherein: X represents a -CH- group or a nitrogen atom; R represents a (C1-C6) alkyl, (C3-C6) cycloalkyl group, in which one or more -CH2- groups may be replaced by -O- and may be substituted with one or more radicals selected from the radicals (C1- C6) alkyl, hydroxy and alkoxy, said compound of formula (I) being in the form of salts.
    [0004] In the composition according to the invention, the compounds of formula (I) may be in the form of basic or acid addition salts compatible with a pharmaceutical use. Among the pharmaceutically acceptable acids, mention may be made, without limitation, hydrochloric, hydrobromic, sulfuric, phosphoric, acetic, trifluoroacetic, lactic, pyruvic, malonic, succinic, glutaric, fumaric, tartaric, maleic, citric, ascorbic, methane- or ethane-sulfonic and camphoric. Among the pharmaceutically acceptable bases, mention may be made, without limitation, of sodium or potassium hydroxide, triethylamine and tert-butylamine. The compounds of formula (I) may have one or more asymmetric centers and may then be isolated in optically active form or in the form of their racemic mixture. Methods for obtaining optically active forms, for example by resolution of a racemic form or by synthesis using racemic starting materials, are well known to those skilled in the art. For the definition of the compounds of formula (I), the term "alkyl" denotes a linear or branched hydrocarbon radical advantageously having 1 to 6 carbon atoms, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert- butyl, pentyl, neopentyl, n-hexyl. The alkyl groups may be substituted by one or more hydroxyl groups and / or by one or more alkoxy groups as defined below. The term "cycloalkyl" refers to a cyclic hydrocarbon system comprising from 3 to 6 carbon atoms and which may be mono- or poly-cyclic. In particular, cyclopropyl and cyclohexyl radicals may be mentioned. The "alkoxy" groups correspond to the linear or branched alkyl groups defined above connected through an -O- (ether) linkage. Particularly preferred are methoxy, ethoxy, n-propyloxy, i-propyloxy, n-butoxy, s-butoxy, t-butoxy, n-pentoxy and propoxy groups. The alkoxy groups may be substituted by an alkyl group as defined above or by another alkoxy group. Among the compounds that can be used as active agents in the compositions according to the invention, there may be mentioned the following compounds of formula (I): 2,3-dihydroxypropyl 2-hydroxybenzoate (coded GPN000136) (S) -2,3-dihydroxypropyl 2-hydroxybenzoate (referenced GPS008506) (R) -2,3-dihydroxypropyl 2-hydroxybenzoate (referenced 10 GPS008505) 2,3-dihydroxypropyl 4-hydroxynicotinate and its isomers dipropylene glycol salicylate and its isomers propylene glycol salicylate and its isomers The invention also relates to a process for preparing the compounds of formula (I), characterized in that, if X = CH in formula (I), a starting acid, which is salicylic acid, is used, and if X = N in the formula (I), a starting acid is used which is 4-hydroxy nicotinic acid; that, initially, the starting acid is activated with an appropriate amount of 1H-benzotriazole in an organic solvent at room temperature in the presence of thionyl chloride; that the activated acid is isolated by filtration and evaporation of the filtrate; that, in a second step, the alcohol obtained, protected or not, is deprotonated by the action of a base in an organic solvent; and that the activated acid isolated in the first time is added at room temperature to yield the compound of formula (I). According to one aspect of the invention, the organic solvent is tetrahydrofuran and the base for deprotonating the alcohol is sodium hydride. According to another aspect of the invention, the activation of the starting acid is carried out with from 3 to 3.5 equivalents of 1H-benzotriazole in the presence of about 1 equivalent of thionyl chloride. According to one aspect of the invention, the composition comprises at least one active agent other than those of formula (I) for exerting at least one complementary or synergistic action. According to another aspect of the invention, the composition according to the invention is in the form of capsules, cream, gel, lotion, milk, emulsion, bi-phasic emulsion, O / W emulsion or W / O emulsion, three-phase emulsion, solution, ointment, body oil, shampoo, soap, lip stick, stick and pencil for make-up, mask, nano-capsule, liposome, transdermal patch for topical applications. In the gel form, the composition may comprise suitable excipients, such as cellulose esters, or other gelling agents, such as the carbopolymeric polymer and guar gum, for example. In the form of emulsions, the compositions according to the invention have a good stability and can be stored for the time necessary for use at temperatures between 0 and 50 ° C, without sedimentation. constituents or phase separation. According to another aspect of the invention, in the composition, the active agent (s) is (are) placed in encapsulation means selected from the group consisting of microspheres, liposomes, glycospheres, chylomicrons, macro-micro-, and nanoparticles, macro-, micro- and nanocapsules. According to another aspect of the invention, in the composition, the active agent (s) is (are) absorbed or adsorbed onto powdery organic polymers, talcs, bentonite or other mineral supports in powder form. According to another aspect of the invention, in the composition, the active agent (s) of formula (I) constitutes (s) from 0.01% to 5% by weight relative to total weight of the composition. According to another aspect of the invention, in the composition, the active agent (s) of formula (I) is (or is) in encapsulated form and is (are) preferably 0.5 to 5% by weight relative to the total weight of the composition. The invention also relates to the use of a composition according to the invention for preventing and / or treating chronic inflammatory diseases either internal, such as Crohn's disease, or external related to seborrheic, acneic, inflammatory and immunological; the composition according to the invention may especially be used to prevent and / or treat dermatoses, psoriasis, atopic dermatitis, as well as inflammatory states of the cutaneous or hair systems inducing itching, irritation, chronic redness or disorders. functional capillary fragility. The invention has thus demonstrated the possibility of using the compounds of formula (I) as active agent (s). These compounds have very good properties for inhibiting certain chemokines, such as CCL22 and / or CCL17. The present invention therefore aims precisely to propose novel pharmaceutical, dermatological or cosmetic compositions capable of treating internal and external chronic inflammatory diseases, such as skin that is irritated, injured or sensitized by external or internal aggression, in general, and dermatoses, in particular particular.
    [0005] Thus, the subject of the present invention is a cosmetic use of at least one derivative of formula (I) for inhibiting the chemokine CCL22 and / or CCL17, for soothing dry skin with an atopic tendency and / or for improving and / or strengthening the skin. hydration of the skin. It therefore concerns applications to treat chronic inflammatory conditions such as Crohn's disease, allergic rhinitis, dermatoses such as atopic dermatitis, eczema and psoriasis.
    [0006] In the therapeutic aim of the invention, the derivatives of formula (I) capable of inhibiting the chemokine CCL22 and / or CCL17 can be administered topically but they can also be administered orally. These derivatives can be used as such in liquid or powder form, not purified or purified. The derivatives of formula (I) capable of inhibiting chemokines may, in the compositions according to the invention, be associated with one another or with other compounds which complement or indeed synergize this effect. The protective activity of the derivatives of formula (I) with respect to free radicals and UV is interesting in the hair field, especially in the case of combination with substances facilitating the good condition of the scalp and hair. It is possible to mention associations with mucopolysaccharides, minerals, vitamins, ceramides, vegetable oils, antiradical substances, U.V. filters, flower acids or fruit acids. Similarly, the repairing activity of the compounds of formula (I) capable of inhibiting chemokines is particularly advantageous when they are associated with substances having a healing effect such as proteins, hyaluronic acid, amino acids, or with anti-inflammatory substances, anti-aging, after-sun anti-acne or anti-dermatosis. Thus, the compositions of the invention are particularly suitable for topical application to prevent and / or treat many skin disorders, especially as a repairing agent and / or protector of the skin and the hair system such as the hair, to fight against external aggressions related to pollution, sun, oxidative stress, aging and cutaneous pathologies leading to dysfunction of the homeostasis of the epidermis or hair. These cosmetic compositions may also take the form of a lotion or solution in which the derivatives according to the invention are in encapsulated form, for example in microspheres. These microspheres may, for example, consist of fat, agar and water. The active agents may also be incorporated into liposome, glycosphere-type vectors in chylomicrons, macro-, micro-, nanoparticles as well as macro-, micro- and nanocapsules and also be adsorbed onto powdery organic polymers, talcs , bentonites and other mineral supports. For the preparation of the compositions according to the invention, the derivatives of formula (I) may be mixed with the excipients generally used in cosmetics. The cosmetic compositions of the invention may therefore contain additives or adjuvants customary in cosmetology, such as, for example, antibacterial agents or perfumes, but also extraction and / or synthetic lipids, gelling and viscosity polymers, tensio- active ingredients, emulsifiers, water-soluble or fat-soluble active ingredients, plant extracts, tissue extracts, marine extracts, or synthetic actives.
    [0007] The dermocosmetic or pharmaceutical use of these derivatives of formula (I) includes all skin and body care products, including sun, protective and tanning products, anti-aging, anti-seborrhoeic, tonic, anti-seborrhoeic products. products that improve the appearance of the skin, including acne treatment, skin redness treatment, scalp treatment and hair loss. The cosmetic compositions of the present invention may also comprise other complementary active agents chosen for their action, for example for sun protection, the anti-wrinkle effect, the antiradical and antioxidant activity, the anti-irritant activity, the cellular nutrition, cellular respiration, cellular hydration and regeneration, anti-seborrhoeic treatments, as well as other active agents having an effect on skin tone and protection of the hair. The cosmetic compositions of the present invention are preferably used daily by applying them one or more times daily. The cosmetic compositions of the present invention are very well tolerated, exhibit no phototoxicity, and their application to the skin for prolonged periods of time does not imply any systemic effects. The invention also relates to the use of derivatives according to the invention for the preparation of pharmaceutical compositions having an anti-inflammatory and / or dermoprotective activity. These compositions are useful for preventing and / or treating in particular dermatological diseases related to seborrheic, acneic, inflammatory and immunological activities.
    [0008] In summary, the invention thus relates to the use of derivatives of formula (I) capable of inhibiting certain chemokines, for the preparation of a pharmaceutical, dermatological or cosmetic composition intended to prevent and / or treat pathologies and resulting disorders: natural or premature aging of the skin or hair; dermatoses, such as psoriasis, acne and atopic dermatitis; inflammatory and / or immune conditions of the cutaneous or hair systems, inducing itching, irritation, chronic redness, irritation and persistent itching, and functional disorders of capillary fragility. The present invention is now illustrated by the examples given below. Examples 1 to 3 illustrate a process for the preparation of derivatives of formula (I) capable of inhibiting the CCL22 chemokine according to the invention. The starting materials are commercially available or can be synthesized by conventional methods known to those skilled in the art. EXAMPLE 1 Synthesis of 2,3-dihydroxypropyl 2-hydroxybenzoate (coded GPN136) OH 0 nzotriazole, S0Cl2, THF Amberlyst CH3OH OHOH 2) solketal, NaH, THF 1 OH GP14000136 In a flask under argon, 2.17 g (3.15 eq) of 1H-benzotriazole are dissolved in 30 mL of anhydrous tetrahydrofuran and then 0.42 mL (1 eq) of thionyl chloride are added. The mixture is stirred for 45 minutes at room temperature and then 800 mg (5.792 mmol, 1 eq) of salicylic acid dissolved in 20 ml of anhydrous tetrahydrofuran are added. The reaction medium is stirred for 3 hours at room temperature. It is then filtered quickly on sintered. The filtrate is evaporated to yield the activated acid (benzotriazol-1-yl (2-hydroxyphenyl) methanone) which is directly engaged in the next step. In a flask under argon, 696 mg (5.266 mmol) of solketal are dissolved in 10 mL of anhydrous tetrahydrofuran and 232 mg (1.1 eq) of sodium hydride (60% in oil) are added. The mixture is stirred for 30 minutes at room temperature. Then 1.1 eq. (5.792 mmol) of benzotriazol-1-yl (2-hydroxyphenyl) methanone dissolved in 20 mL of anhydrous tetrahydrofuran are added. The reaction medium is stirred for 5 hours at room temperature and then hydrolysed with 30 ml of water. It is extracted with dichloromethane (3 times 30 mL). The combined organic phases are dried over magnesium sulfate, filtered and evaporated under reduced pressure. The crude is purified by chromatography on silica gel (petroleum ether / ethyl acetate 9/1) to give 1.16 g (yield: 87%) of the expected product coded 1 in the form of a colorless oil. 1H NMR (250 MHz, CDCl3): 1.39 (s, 3H, CH3); 1.46 (s, 3H, CH,); 3.89 (dd, 1H, J = 5.3 and 8.7 Hz, CH 2); 4.16 (dd, 1H, J = 6.2 and 8.7 Hz, CH 2); 4.33 - 4.51 (m, 3H, CH 2 and CH); 6.89 (td, 1H, J = 1.2 and 8.0 Hz, CH Ar); 6.99 (dd, 1H, J 0.9 and 8.3 Hz, CH Ar); 7.47 (td, 1H, J - 1.6 and 8.3 Hz, CH 3 Ar); 7.87 (dd, 1H, J = 1.6 and 8.0 Hz, CH Ar); 10.62 (s, 1H, OH). In a flask, 1 g (3.9640 mmol) of coded Compound 1 is dissolved in 25 mL of methanol and 1.2 g of activated Amberlyst 15 resin are added. The medium is stirred for 19 hours at room temperature, filtered on sintered. The resin is rinsed with methanol. The filtrate is then evaporated under reduced pressure. The crude product is purified by chromatography on silica gel (petroleum ether / ethyl acetate: 5/5 then 3/7) to give 620 mg of the coded compound GPN000136 (yield: 74%) in the form of a white solid. . 1H NMR (250 MHz, CDCl3): δ 2.61 (sl, 1H, OH); 3.07 (sl, 1H, OH); 3.68 - 3.78 (m, 2H, CH 2); 4.05 - 4.15 (m, 1H, CH); 4.39 - 4.51 (m, 2H, CH 2); 6.88 (td, 1H, J = 1.0 and 7.6 Hz, CH Ar); 6.98 (dd, 1H, J = 0.9 and 8.4 Hz, CH Ar); 7.46 (td, 1H, J = 1.7 and 7.7 Hz, CH Ar); 7.84 (dd, 1H, J = 1.7 and 8.0 Hz, CH Ar); 10.57 (s, 1H, OH).
    [0009] NMR 130 (62.5 MHz, CDCl3): 63.5 (CH2); 65.6 (OH2); 70.2 (CH); 112.1 (Cq); 117.9 (CH); 119.4 (CH); 130.0 (CH); 136.2 (CH); 161.8 (Cq); 170.3 (Cq). Example 2 Synthesis of (S) -2,3-dihydroxypropyl 2-hydroxybenzoate (coded GPS008506) Amberlyst CH3OH OH OH OMM1) benzotriazole, S0C12, THFH2) NaH, THF OH 0 roe (0) 074_, 2 In a flask under argon, 0.3 mL (2.4194 mmol) of (R) -2,3-O-isopropylidene glycerol are dissolved in 5 mL of anhydrous tetrahydrofuran and 106 mg (1.1 eq) of sodium hydride ( 60% in oil) are added The mixture is stirred for 30 minutes at room temperature Then 637 mg (1.1 eq) of previously described activated acid (benzotriazol-1-yl (2-hydroxyphenyl) methanone) dissolved in 12 ml of anhydrous tetrahydrofuran are added The reaction medium is stirred for 4 hours at room temperature and then hydrolysed with 40 ml of water and extracted with dichloromethane (3 times 30 ml) The combined organic phases are dried over magnesium sulphate, filtered and evaporated under reduced pressure The crude is purified by chromatography on silica gel (p-diethyl ether). Trole / ethyl acetate 9/1) to afford 520 mg (yield: 85%) of the expected product (coded 2) as a colorless oil.
    [0010] 1H NMR (250 MHz, CDCl3): 1.39 (s, 3H, CH3); 1.46 (s, 3H, CH3); 3.89 (dd, 1H, J = 5.3 and 8.7 Hz, CH 2); 4.16 (dd, 1H, J = 6.2 and 8.7 Hz, CH 2); 4.33 - 4.51 (m, 3H, CH 2 and CH) 6.89 (td, 1H, J = 1.2 and 8.0 Hz, CH Ar); 6.99 (dd, 1H, J = 0.9 and 8.3 Hz, CH Ar); 7.47 (td, 1H, J = 1.6 and 8.3 Hz, CH 15 Ar); 7.87 (dd, 1H, J = 1.6 and 8.0 Hz, CH Ar); 10.62 (s, 1H, OH). In a flask, 650 mg (2.5766 mmol) of coded compound 2 are dissolved in 10 mL of methanol and 800 mg of activated Amberlyst 15 resin are added. The medium is stirred for 20 hours at room temperature, filtered through sintering. The resin is rinsed with methanol. The filtrate is then evaporated under reduced pressure. The crude is purified by chromatography on silica gel (petroleum ether / ethyl acetate: 6/4, 4/6 and then 3/7) to give 440 mg of the compound referenced GPS008506 (yield: 80%) in the form of of a white solid. Rotatory power: ofi, = +15.5 (c = 1; Et0H). 1H NMR (250 MHz, CDCl3): δ 2.61 (si, 1H, OH); 3.07 (si, 1H, OH); 3.68 - 3.78 (m, 2H, CH 2); 4.05 - 4.15 (m, 1H, 30 CH); 4.39 - 4.51 (m, 2H, CH 2); 6.88 (td, 1H, J = 1.0 and 7.6 Hz, CH Ar); 6.98 (dd, 1H, J = 0.9 and 8.4 Hz, CH Ar); 7.46 (td, 1H, J = 1.7 and 7.7 Hz, CH Ar); 7.84 (dd, 1H, J = 1.7 and 8.0 Hz, CH Ar); 10.57 (s, 1H, OH).
    [0011] NMR (62.5 MHz, CDCl3): 63.5 (CH2); 65.6 (CH2); 70.2 (CH); 112.1 (Cq); 117.9 (CH); 119.4 (CH); 130.0 (CH); 136.2 (CH); 161.8 (Cq); 170.3 (Cq).
    [0012] Example 3 Synthesis of (R) -2,3-dihydroxypropyl 2-hydroxybenzoate (coded GPS008505) In a flask under argon, 0.4 ml (3.2385 mmol) of (S) -3, 3-O-isopropylidene glycerol are dissolved in 7 mL of anhydrous tetrahydrofuran and 142 mg (1.1 eq) of sodium hydride (60% in oil) are added. The mixture is stirred for 30 minutes at room temperature. Then 852 mg (1.1 eq) of previously described activated acid (benzotriazol-1-yl (2-hydroxyphenyl) methanone) dissolved in 16 mL of anhydrous tetrahydrofuran are added. The reaction medium is stirred for 5 hours at room temperature and then hydrolyzed with 40 ml of water. It is extracted with dichloromethane (3 times 30 mL). The combined organic phases are dried over magnesium sulphate, filtered and evaporated under reduced pressure. The crude is purified by chromatography on silica gel (petroleum ether / ethyl acetate 9/1) to give 730 mg (yield: 89%) of the expected product (coded 3) in the form of a colorless oil.
    [0013] 1 H NMR (250 MHz, CDCl 3): δ 1.39 (s, 3H, CH 3); 1.46 (s, 3H, CH3); 3.89 (dd, 1H, J = 5.3 and 8.7 Hz, CH 2); 4.16 (dd, 1H, J - 6.2 and 8.7 Hz, CH 2); 4.33 - 4.51 (m, 3H, CH 2 and CH); 6.89 (td, 1H, J = 1.2 and 8.0 Hz, CH Ar); 6.99 (dd, 1H, J = 0.9 and 8.3 Hz, CH Ar); 7.47 (td, 1H, J - 1.6 and 8.3 Hz, CH 2) NaH. ## STR1 ## 1) benzotriazole, SOCI THF OH (OH Ar); 7.87 (dd, 1H, J - 1.6 and 8.0 Hz, CH Ar); 10.62 (s, 1H, OH). In a flask, 650 mg (2.5766 min) of coded compound 3 are dissolved in 15 mL of methanol and 800 mg of activated Amberlyst 5 resin are added. The medium is stirred for 24 hours at room temperature, filtered on sintered. The resin is rinsed with methanol. The filtrate is then evaporated under reduced pressure. The crude is purified by chromatography on silica gel (petroleum ether / ethyl acetate: 5/5 and then 3/7) to give 480 mg of the compound referenced GPS008505 (yield: 87%) in the form of a solid. White. Rotatory power: ap = -16.6 (c = 1; Et0H). 1 H NMR (250 MHz, CDCl 3): δ 2.61 (si, 1H, OH); 3.07 (si, 1H, OH); 3.68 - 3.78 (m, 2H, CH 2); 4.05 - 4.15 (m, 1H, 15 CH); 4.39 - 4.51 (m, 2H, CH 2); 6.88 (td, 1H, J = 1.0 and 7.6 Hz, CH Ar); 6.98 (dd, 1H, J = 0.9 and 8.4 Hz, CH Ar); 7.46 (td, 1H, J = 1.7 and 7.7 Hz, CH Ar); 7.84 (dd, 1H, J = 1.7 and 8.0 Hz, CH Ar); 10.57 (s, 1H, OH). NMR 130 (62.5 MHz, CDCl3): 63.5 (CH2); 65.6 (OH2); 70.2 (CH); 112.1 (Cq); 117.9 (CH); 119.4 (CH); 130.0 (CH); 136.2 (CH); 161.8 (Cq); 170.3 (Cq). Example 4 demonstrates the technical protocol for recording the effects of the products according to the invention on chemokines. Example 4: Inhibitory properties of the derivatives of formula (I) on chemokines. Cells Expressing the CCR4 Receptor of the Chemokines In order to allow easy recording of CCR4 receptor associated responses, the cDNA that allows its expression is cloned into the plasmid pIRES (ClonTech) in fusion with the GFP protein, according to Vollmer et al. (1999), which makes it easy to measure the expression of the EGFP-CCR4 chimeric receptor. The HEK 293 (ATCC) cells are cultured in MEM minimum essential medium (Invitrogen) in the presence of 10% fetal calf serum (Gibco-BRL), 100 U / ml penicillin (Invitrogen), 100 μg / ml streptomycin (Invitrogen) ) and 2 mM L-glutamine (Invitrogen) at 37 ° C in an atmosphere saturated with water and containing 5% CO 2. The cells are transfected according to the calcium phosphate precipitate method of Chen and Okayama, 1986, and the cells having incorporated the expression plasmid are selected using 600 ag / ml of G-418 geneticin (PAA). for 5 weeks. The clones are selected after being characterized by fluorescence microscopy and fluorescence-assisted cell sorting (FACS) cytometric analysis. Construction of the Gqi5 Recombinant Protein In order to allow easy recording of cellular responses associated with the CCR4 receptor, its natural coupling to the inhibition of cAMP production is changed to phospholipase C coupling which allows for calcium measurements. For this purpose, the chimeric protein Gq15 is produced by site-directed mutagenesis of the last 5 amino acids of Gq, which are replaced by their Gi counterparts. Cells expressing the CCR4 receptor are transfected with plasmid pCNA3.1 in which the cDNA encoding Gq15 was incorporated. Recordings of calcium responses are made the day after transfection. Registration of Cellular Calcium Responses and Their Inhibition by GPN Collection Molecules: Cells are loaded with INDO-1 fluorescent calcium indicator, according to the protocol provided by the supplier (Molecular probes), distributed in microplate wells and placed in a fluorescence reader-pipettor (FlexStation, Molecular devices). The elevation of intracellular calcium is measured by changing fluorescence intensity at 401 and 475 nm (excitation 355 nm). Measuring points are recorded every 5s for 150s for each well. The molecules are tested in the presence of 5 nM chemokine CCL22 (commercial) Figure 1 generally illustrates the results obtained: the addition of chemokine (CK) to cells expressing the chemokine receptor (CKR) triggers a release of intracellular calcium harbored by fluorescence change in the Indo-1 calcium probe. The response develops in seconds and decreases over time to return in about 2 minutes at a concentration of 5 to 10 nM CCl22 chemokine on HEK 293 cells expressing CCR4. If the molecules to be tested are preincubated with the chemokine [CK + N] and the mixture is added to the cells, a marked decrease in the amplitude of the response is observed if the test molecule is a neutralizer of the chemokine. Conversely, when the neutraligand is preincubated with the cells expressing the [CKR + N] receptor, a much smaller decrease in the amplitude of the response is observed. This protocol was used to identify the CCL22 neutraligands shown in Table 1. The apparent affinity of GPN136 molecules is determined by establishing a dose-response relationship of inhibition of calcium response as well as presented in FIG. 2 and the results obtained are reported in table 1.
    [0014] TABLE 1 Structure / Function Relations of the Derivatives of the Formula (I) TABLE 1 Reference Structure Inhibition Inhibition of the CCL17-induced calcium calcium response responses (%) CCL22 (%) GPN136 OH 0 0 35 ± 8 o'-y- GPS008504 OH 0 0 OH 0 10 ± 5, - o o ofi GPS008505 OH 0 0 37 ± 6 i 011 GPS008506 OH 0 0 40 ± 5 o o o GPS008507 o 0 0 00H CeH GPS008508 110 0 0 0 ## STR1 ## The analysis of the selectivity of the molecules is carried out by measuring the inhibition of the response evoked by other chemokines on their receptors. Thus, in FIG. 3, the molecules were tested on the CCL2 / CCR2, CCL3-5 / CCR5, CCL17 / CCR4, CCL22 / CCR4, CXCL8 / CXCR8, CXCL10 / CXCR2, CXCL11 / CXCR3, CXCL12 / CXCR4 pairs. Figure 3 gives the selectivity of CCL22 neutraligands (GPN136 (black bars) and GPN025 (white bars)) on the indicated chemokines and their respective receptors. Determination of the interaction affinity of CCL22 neutraligands by quenching fluorescence of tryptophan residues. This measurement is carried out as described in Hachet-Haas et al. (Small neutralizing molecules to inhibit actions of the chemokine CXCL12, J Biol Chem, 2008, 283 (34), 23189-99). Briefly: the fluorescence measurements are made on a Fluorolog 3 spectrofluorometer (JobinYvon / Spex) in a quartz cuvette. CCL22 (1.5 μM (HEPES buffer without albumin) in 1 mL final volume) is excited at 285 nm and its emission spectrum is recorded between 300 and 400 nm after each addition of an aliquot of test molecule (1 μL ). The measurements are carried out at 20 ° C. and the solutions are stirred using a magnetic bar. The recorded emission spectra are corrected by subtracting the emission spectrum of the test compound alone. Figure 4 indicates that fluorescence quenching of CCL22 is observed only with the neutraligand (GPN 136) and not with the receptor antagonist (C-021). The shape of the titration curve of CCL22 (1.5 μM) by GPN 136 is biphasic, indicating the likely presence of several GPN binding sites 136, at least one of which is of high affinity. The adjustment of the traces by the equation (RL) 2+ (RL) x (-Ro-Lo-KD) + RoxLo = 0 with (RL) = ((Ro + Lo + KD) ± ((- Ro-Lo -KD) 2 -4xRoxLo) 1/2) / 2 allows to estimate the affinity for each site: KD <50 nM for the high affinity site and KD = 53pM. There is no signal for the C-021 molecule. Figure 4 shows the inhibition of the intrinsic fluorescence of CCL22 by the neutraligand GPN 136 (white squares). The CCR4 receptor antagonist, C-021, does not show a decrease in tryptophan fluorescence (black squares).
    [0015] Example 5 shows how non-cytotoxic active candidates are selected. Example 5 Properties of Derivatives on Keratinocyte Migration and Cytotoxicity The activity of the GPN 136 molecule was evaluated in a human keratinocyte migration assay performed according to a cell-wall healing test. It is indeed known that in these chronic pathologies, a large migration of cells can promote the formation of pruritus, one of the manifestations of dermatitis. Immortalized human keratinocytes obtained from DKFZ (Heidelberg, Germany) are cultured in Eagle-modified Dulbecco's medium (DMEM, InVitrogen) supplemented with 10% fetal calf serum (Gibco-BRL) and 100 U / ml penicillin. (Invitrogen), 100 μg / ml streptomycin (Invitrogen) and 2 mM L glutamine (Invitrogen) at 37 ° C and 5% CO2. The wells of a 24-well culture plate are inoculated with 200,000 cells and the culture is maintained until a confluent mat is formed. The carpet is then damaged by performing a linear scraping using sterile 0.1-10pL pipette tip. The medium is then replaced to remove the cells and their debris and the culture is continued under different experimental conditions: (i) without any addition, (ii) addition of 100 ng / ml of CCL22 and (iii) addition of a mixture containing 100 ng / ml of CCL22 and the molecule to be tested, for example GPN 136. The effect of the molecules alone is also recorded in different wells. In order to block cell proliferation, each well receives a dose of lpg / ml of mytomycin (Sigma-Aldrich). The culture plates are placed in the IncuCyte device, which records the growth of the cell layer by imaging transmitted under cell culture conditions (37 ° C., 5% CO2, saturated atmosphere of water) for 72 hours. Phase contrast images are acquired every 2 hours. Healing is defined (quantified) as the ratio of scar width at time t to time O. The experiment is repeated three times per condition. The results obtained are reported in FIG. 5. FIG. 5 shows the migration of human keratinocytes under the effect of GNP136; it shows photographs of human Hacat cell carpets after injury at time 0 and after 72h of culture in the presence of excipient alone (DMSO, panel 1), CCL22 and excipient (CCL22 + DMSO, panel 2) and CCL22 and GPN 136 (CCL22 + GPN136, panel 3) The figure indicates that cell migration is strongly accelerated by the CCL22 chemokine which acts on CCR4 receptors present in Hacat cells. This effect of the chemokine CCL22 is abolished by the molecule GPN136. Some compounds are cytotoxic: example of the GPN 025 molecule. FIG. 6 shows the effect of the GPN025 molecule added on the OJ day after injury of the Hacat cell mat. It is noted that the cells appear to have formed syncytia after 72 hours of incubation. It is impossible to distinguish the outlines. The molecule GPN025 damages Hacat cells. This effect is quantified in the metabolic activity experiment shown in Figure 6 The metabolic activity test is a colorimetric test (WST-1 assay - Ozyme) based on measuring the activity of the mitochondrial enzyme succinate -tetrazolium reductase cleaving the tetrazolium salt WST-1 ((4- [3- (4-Iodophenyl) -2- (4-nitrophenyl) -2H-5-tetrazolol] -1,3 benzene disulfonate)) to a water-soluble derivative , formazan which is a light absorbing dye in the 420-480 nm window. HaCat cells are distributed at 200,000 cells per well in a 24-well culture plate, in a final volume of 500 μl. They are cultured for 24 hours before adding mitomycin C (1 g / ml) and the test compound. The culture plate is incubated for 72 hours at 37 ° C., 5% CO 2, in a saturated atmosphere of water. The medium is then replaced with fresh medium supplemented with WST-1 reagent and then incubated for 2 hours at 37 ° C. The plates are shaken to homogenize the color in the wells and the absorbance is measured at 450 nm in a multiwell plate reader. The measurements are made in triplicates. The enzymatic activity requiring cellular integrity, the absorbance will be even stronger than the metabolic state of the cells is good. Figure 7 shows the cytotoxic activity of GPN136 and GPN025. In vitro cytotoxic activity: HaCat cell cultures are treated with different molecule concentrations: 0.3 μM (dark gray bars) 10 μM (light gray bars) or 30 μM ( white bars) of the GPN 136 and GPN 025 molecules. The activity of the mitochondial enzyme formazan reductase is recorded using the WST-1 chromogenic substrate which is transformed into a colored product, formazan, detected at 450 nm. As shown in FIG. 7, the GPN 136 molecule does not affect the enzymatic activity. On the other hand, the GPN 025 molecule reduces dose-dependently and significantly cellular metabolism, thus revealing a cytotoxic effect of GPN 025.
    [0016] Example 6 This example shows a formulation of the composition according to the invention. COMMERCIAL NAME INCI NAME PERCENTAGE IN FORMULA Simulsol 165 PEG-100 Stearate / Glyceryl Stearate 3 Montanov L C14-22 Alcohol / C12-20 Alkyl Glucoside 2 Safflower Oil Carthamus Tinctorius Seed Oil 2 Shea Butter Butyrospermum Parkii Butter 4 Ceramidone Octyldodecyl PCA 3 Phytosqualane Squalane 5 DUB MCT 5545 Caprylic / Capric Triglycerides 7 Beeswax Cera Alba 1 Sorbic Acid Sorbic Acid 0.1 Glycerin Glycerin 2 Sorbitol Sorbitol 3 Aristoflex AVC Ammonium 1.3 Acryloyldimethyltaurate / VP copolymer Xanthan gum Xanthan gum 0.2 Nicotinamide Niacinamide 1 Lipacid C8G Capryloyl glycine 0.5 Aqua Qsp purified water for 100 GPN136 - 3 This composition is applied every day for 1 month on the facial skin of a 20 year old man with atopic dermatitis on the whole face; this application is made at a rate of about 2 g / day for the complete face. The total disappearance of the inflammation zones at the end of the treatment is observed. The following have been proposed ingredients that can be combined with the compositions for topical application in order to amplify the effect or achieve action synergies, especially with the formulation of this example 6 (the amounts are given in percentage by weight) a) ingredients with anti-inflammatory properties commonly used in cosmetics including nicotinamide (1 to 4%), 18 beta glycyrrhetinic acid (0.01 to 1%), alpha bisabolol (0.1 to 1%) ; b) glycine having a soothing effect (from 1 to 3%); c) the silver micro-particles (0.1%) which make it possible to regulate, stabilize the microbial flora and avoid superinfection; d) a bacterial anti-adhesion agent to limit the adhesion and proliferation of Staphylococcus aureus such as Solabia Teflose composed of propanediol, rhamnose, glucose and glucuronic acid (2-4%); e) unsaponifiables of shea butter and sunflower to reinforce the skin barrier and improve skin hydration. (0.5 to 2%). f) a chicory extract of 1 to 3% (as the Vederin Silab) for strengthening and recovery of the skin barrier; g) a derivative of raspberry seed oil and vitamin E succinate (1% Raspberry seed oil / Tocopheryl succinate / Aminopropanediol esters) such as Vitaskin® E from Solabia; h) octyldodecyl PCA assayed at 0.5 to 5% to stimulate the synthesis of epidermal lipids; j) a safflower or evening primrose vegetable oil stabilized in a ceramide-like form such as "Omega 6 Ceramide® Safflower" and "Omega 6 Ceramidee Evening Primrose" by Solabia to improve cellular cohesion (0.1 to 1%); j) an optimized blend of argan oil, shea butter and barley wax, as in DSM's Stimutex® AS, for anti-inflammatory action and to decrease histamine release (2). at 5%); k) a mixture of white birch bark extract and Scrophularia nodosa, as in Greentech Protectol®, for its anti-inflammatory properties due to the presence of betulinic acid (1 and 3%); 1) a mixture of lactose and 0.5% milk protein as in CLR NodukineTM for its anti-inflammatory properties.
    权利要求:
    Claims (21)
    [0001]
    REVENDICATIONS1. Dermato-cosmetic or pharmaceutical composition usable for treating inflammatory diseases of human or animal skin, characterized in that it contains, in a pharmaceutically acceptable vehicle, an effective amount of at least one active agent consisting of a compound of formula ( I): wherein: X is -CH- or nitrogen; R represents (C1-C6) alkyl, (C3-C6) cycloalkyl, in which one or more -CH2- groups may be replaced by -O- and may be substituted by one or more groups selected from radicals (C1); -C6) alkyl, hydroxy and alkoxy, the compound of formula (I) may be in the form of salts. 20
    [0002]
    2. Composition according to claim 1, characterized in that it contains at least one compound of formula (I) in the form of basic or acid addition salt compatible with a pharmaceutical use.
    [0003]
    3. Composition according to claim 2, characterized in that, when a compound of formula (I) is present in salt form, it constitutes an acid addition salt, said acid being selected from the group formed by the acids hydrochloric, hydrobromic, sulfuric phosphoric, oacetic, trifluoroacetic, lactic, pyruvic, malonic, succinic, glutaric, fumaric, tartaric, maleic, citric, ascorbic, methane- or ethanesulfonic and camphoric.
    [0004]
    4. Composition according to claim 2, characterized in that, when the compound of formula (I) is present in salt form, it constitutes a basic addition salt, said base being chosen from the group formed by the hydroxide of sodium or potassium, triethylamine or tert-butylamine.
    [0005]
    5. Composition according to one of claims 1 to 4, characterized in that, in the formula of (or) compound (s) of formula (I) it contains, the (or) alkyl radical (s) is (are) chosen from the group consisting of methyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl, neopentyl and n-hexyl radicals, which may or may not be substituted by one or more hydroxyl groups or by a or more alkoxy groups.
    [0006]
    6. Composition according to one of claims 1 to 5, characterized in that, when a compound of formula (I) has an R cycloalkyl radical, said radical is mono or polycyclic, especially cyclopropyl or cyclohexyl.
    [0007]
    7. Composition according to one of claims 1 to 6, characterized in that, when a compound of formula (I) contains in its formula at least one alkoxy radical, said alkoxy radical is chosen from the group formed by the groups methoxy, ethoxy, n-propyloxy, i-propyloxy, n-butoxy, s-butoxy, t-butoxy, n-pentoxy and s-pentoxy, each alkoxy group being alkyl-substituted as defined in claim 5 .
    [0008]
    8. Composition according to one of claims 1 to 7, characterized in that the compound of formula (I) is chosen from the group formed by 2,3-dihydroxypropyl 2-hydroxybenzoate (S) -2,3-dihydroxypropyl 2-hydroxybenzoate (R) -2,3-dihydroxypropyl 2-hydroxybenzoate 2,3-dihydroxypropyl 4-hydroxynicotinate and its isomers dipropylene glycol salicylate and its isomers propylene glycol salicylate and its isomers
    [0009]
    9. Composition according to one of claims 1 to 8, characterized in that it contains from 0.01 to 5% by weight of compound (s) of formula (I) relative to the total weight of the composition.
    [0010]
    10. Composition according to one of claims 1 to 9, characterized in that the (or) active agents of formula (I) is (are) in powder form, or are absorbed on powdery organic polymers, in particular talcs or Bentonites, or are in encapsulated form.
    [0011]
    11. Composition according to claim 10, characterized in that the means for encapsulation of the agent (or agents) active (s) of formula (I) is (or are) chosen from the group formed by microspheres, liposomes, glycospheres, chylomicrons, macromicro-, and nanoparticles, macro-, micro- and nanocapsules.
    [0012]
    12. Composition according to one of claims 1 to 11, characterized in that it contains at least one active agent 25 other than a compound of formula (I) taken from the group formed by sun protection agents, the agents antirides with anti-radical activity, antioxidant, anti-irritant, agents promoting cellular nutrition, cellular respiration, cellular hydration, cell regeneration, anti-seborrhoeic treatments, cutaneous tonicity, protection of the hair, cicatrizing agents , hyaluronic acid, amino acids, anti-aging agents and after-sun agents.
    [0013]
    13. Composition according to one of claims 1 to 12, characterized in that it is formulated in the form of lotion, gel, emulsion, cream or milk, two-phase emulsion oil in water or water in oil, tri-phase emulsion , body oil, shampoo, soap, mask, ointment, ointment and pencil for makeup, lipstick, nanocapsule, liposome or transdermal patch for topical applications.
    [0014]
    14. Composition according to one of claims 1 to 13, characterized in that it contains mucopolysaccharides, vitamins, ceramides, vegetable oils and agents effective in dermatoses.
    [0015]
    15. Composition according to one of claims 1 to 14, characterized in that it contains antibacterial agents, perfumes, extraction and / or synthetic lipids, gelling and viscosity polymers, surfactants, emulsifiers, plant extracts, tissue extracts, marine extracts, hydro- or lipo-soluble active agents, synthetic actives. 20
    [0016]
    16. Composition according to one of claims 1 to 15, characterized in that it is administrable topically or orally.
    [0017]
    17. Composition according to one of claims 1 to 16, for use on an animal or human subject, in the treatment of dermatological diseases related to seborrheic, acneic, inflammatory and immunological activities.
    [0018]
    18.Composition according to claim 17, for use in the treatment of chronic inflammatory diseases or external, including dermatoses, acne, atopic dermatitis, psoriasis, eczema, dry skin atopic tendency, redness of the skin, either internal, including Crohn's disease, asthma and allergic rhinitis.
    [0019]
    19. Process for the preparation of the compounds of formula (I) as defined in claim 1, characterized in that, if X = CH in formula (I), a starting acid, which is salicylic acid, is used, and if X = N in the formula (I), a starting acid is used, which is 4-hydroxy nicotinic acid; that, initially, the starting acid is activated with an appropriate amount of 1H-benzotriazole in an organic solvent at room temperature in the presence of thionyl chloride; that the activated acid is isolated by filtration and evaporation of the filtrate; that, in a second step, the alcohol obtained, protected or not, is deprotonated by the action of a base in an organic solvent; and that the activated acid isolated in the first time is added at room temperature to yield the compound of formula (I).
    [0020]
    20. Process according to claim 19, characterized in that the organic solvent is tetrahydrofuran and the base for deprotonating the alcohol is sodium hydride. 20
    [0021]
    21. The method of claim 20, characterized in that the activation of the starting acid is carried out with from 3 to 3.5 equivalents of 1H-benzotriazole in the presence of about 1 equivalent of thionyl chloride.
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    同族专利:
    公开号 | 公开日
    US20170281653A1|2017-10-05|
    FR3026011B1|2019-07-19|
    EP3197460A1|2017-08-02|
    WO2016046456A1|2016-03-31|
    引用文献:
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    WO2013083825A1|2011-12-08|2013-06-13|Pierre Fabre Dermo-Cosmetique|New lipogenesis inhibitor compounds|
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    WO2003013548A1|2001-08-09|2003-02-20|Sekisui Chemical Co., Ltd.|Medical composition for external use for dermatosis|CN106749211B|2016-11-22|2019-08-09|斯芬克司药物研发（天津）股份有限公司|A kind of nicotinate and the preparation method and application thereof|
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    优先权:
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    FR1402163A|FR3026011B1|2014-09-24|2014-09-24|COMPOSITION COMPRISING AT LEAST ONE INHIBITOR OF CERTAIN CHEMOKINES, METHOD FOR PRODUCING THE SAME AND USE THEREOF DERMOCOSMAL PHARMACEUTICAL|
    FR1402163|2014-09-24|FR1402163A| FR3026011B1|2014-09-24|2014-09-24|COMPOSITION COMPRISING AT LEAST ONE INHIBITOR OF CERTAIN CHEMOKINES, METHOD FOR PRODUCING THE SAME AND USE THEREOF DERMOCOSMAL PHARMACEUTICAL|
    EP15788100.4A| EP3197460A1|2014-09-24|2015-09-22|Dermocosmetic or pharmaceutical use of a composition containing at least one inhibitor of certain chemokines|
    PCT/FR2015/000185| WO2016046456A1|2014-09-24|2015-09-22|Dermocosmetic or pharmaceutical use of a composition containing at least one inhibitor of certain chemokines|
    US15/514,227| US20170281653A1|2014-09-24|2015-09-22|Dermocosmetic or pharmaceutical use of a composition containing at least one inhibitor of certain chemokines|
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